Membership of EANNASO is made up of National Networks of AIDS Service Organisations in the Eastern Africa region. There are two categories of membership namely:-

Ordinary members of EANNASO are the National Networks of AIDS Organisations who became members in their own right by virtue of being the National Networks in the member country.

Associate membership of EANNASO is constituted by regional thematic networks, who apply for and are accepted for membership.

Application for Associate Membership is made in writing to the EANNASO board Chairperson. Once the letter is received it is discussed in a board sitting and approved by the EANNASO Board and ratified by the General Assembly

The Board extends honorary membership to corporate bodies, prominent individuals or any other associations in the world who have made a positive contribution to the regional or otherwise in matters related to HIV/AIDS prevention, care and support.